平滑肌细胞膜超极化在过氧亚硝酸阴离子降低大鼠血管反应性中的作用

摘要

本工作对ONOO<'->引起血管反应性下降的机理进行了详细的探讨.1)ONOO<'->可以促使小动脉对去甲肾上腺素的收缩反应性下降:2)一定量的ONOO<'->可以引起ASMCs超极化和细胞内钙降低,带来反应性下降:3)ONOO<'->激活BK<,Ca>通道引起STOCs的活动增强,导致细胞出现超极化;4)ONOO<'->通过激活VGCCs和增强RyRs的活动引起BK<,Ca>通道开放和STOCs活动增强;5)大剂量的ONOO<'->引起ASMCs持续性去极化和细胞内钙水平上升;可能参与了钙超载的发生;本工作查明;一定量的ONOO<'->引起ASMCs超极化;参与了低血管反应性的发生;大剂量ONOO<'->引起ASMCs去极化,参与了钙超载的发生.对其作用机理的阐明,为防治NO和ONOO<'->引起细胞损伤提供了新的思路.

目录

ABBREVIATIONS

ABSTRACT (IN CHINESE)

ABSTRACT (IN ENGLISH)

SECTION I. PEROXYNITRITE INDUCES ARTERIOLAR SMOOTH MUSCLE CELL MEMBRANE HYPERPOLARIZATION WITH ARTERIOLAR HYPOREACTIVITY IN RATS

1. Introduction

2. Materials and Methods

3. Results

4. Discussion

5. References

附录

SECTIONⅡ. MOBILIZATION OF INTRACELLULAR CALCIUM BY PEROXYNITRITE IN ARTERIOLAR SMOOTH MUSCLE CELLS FROM RATS

1. Introduction

2. Materials and Methods

3. Results

4. Discussion

5. References

附录

SECTION Ⅲ CALCIUM MECHANISMS UNDERLYING PEROXYNITRITE-MEDIATED ENHANCEMENT OF SPONTANEOUS TRANSIENT OUTWARD CURRENT IN RAT ARTERIOLAR SMOOTH MUSCLE CELL

1. Introduction

2. Materials and Methods

3. Results

4. Discussion

5. References

SUMMARY AND PERSPECTIVE

REVIEW

PUBLICATIONS

ACKNOWLEDGEMENT