Quantification System of Ionic Dissociative Materials in Glycolytic Pathway Using Infrared Spectroscopic Information

摘要

We provided a method of infrared spectral extraction of ionic dissociative materials under the assumption of their ionic dissociation equilibrium, and it became possible to quantify their content in the aqueous solutions. Additionally, we constructed a simultaneous quantification system of the concentrations of the ionic dissociative materials in their mixture solution and examined to apply it to the enzyme reaction system in glycolytic pathway. In the present work, we focused our attention on the enzyme reaction from glucose 6-phosphate (G6P) to fructose 6-phosphate (F6P) with D-glucose-6-phosphate ketol-isomerase, and all infrared spectra were collected with a Fourier transform infrared (FT-IR) spectrometer equipped with a horizontal diamond ATR sampling accessory. This enzyme reaction stands on the entrance of glycolytic pathway, which would administer dynamics of the overall metabolism. Then this reaction might be appreciated as one of the most important biological reaction in experimental and computational metabolic engineering.