SURFACE-FUNCTIONALIZED SILK FIBROIN FILM WITH CONTROLLABLE CELL ADHESION BY SURFACE-INITIATED ATOM TRANSFER RADICAL POLYMERIZATION

摘要

Surface-initiated atom-transfer radical polymerization (ATRP) was employed to tailor the functionality of silk fibroin (SF) film surface in a well-controlled manner. Functional polymer brushes of N-isopropylacrylamide (NIPAAm) and 2-hydroxyethyl methacrylate (HEMA) were prepared via surface-initiated ATRP from the SF films. Surface cultures of the 3T3 cell line on the functionalized SF films were evaluated. At 37 °C, the seeded cells adhered, spread, and proliferated on the SF films grafted by P(NIPAAm) and P(HEMA)-b-P(NIPAAm). Below the LCST, the cells detached from the SF-g-P(NIPAAm) surface spontaneously. SF films grafted with P(HEMA) and P(NIPAAm)-b-P(HEMA) brushes showed similar cell attachment/detachment with that of P(HEMA) modified SF films, respectively. These functionalized SF films can be potentially useful as SF-based biomedical materials, such as wound dressing, wound healing and cell cultivation substrates.