IR-Spectroscopic imaging a

摘要

Abstract: Tissue samples from colon biopsy have been analyzed using FT-IR microscopy. For differentiation of cancerous versus normal tissue and for imaging of tissue structure linear and quadratic discriminant analysis was applied. A large number of samples has been used for statistical parametrization. After IR mapping, the samples have been analyzed with common pathological techniques. Comparing the pathological classification with the results of the discriminant analysis the resolving power of the later is estimated. The influence of filtering (using derivative spectra, baseline correction, and normalization) has been investigated. In vivo application of IR-spectroscopy using IR laser diodes or grating spectrometers for fast measurements will lead to a reduction of data concerning spectral range and resolution. The accompanying loss in differentiation of tissue is discussed. Images constructed with the discriminant analysis are compared to corresponding images from visible microscopy.!10