Effect of Laser Fluence in Laser-Assisted Direct Writing of Human Colon Cell

摘要

Matrix-Assisted Pulsed-Laser Evaporation Direct-Write (MAPLE DW) has been emerging as a promising biological construct fabrication technique. The post-transfer cell viability in MAPLE DW depends on various operation conditions such as the applied laser fluence. For wide implementation of MAPLE DW-based biofabrication, the effect of laser fluence on the post-transfer cell viability should be first carefully understood. This study investigates the effect of laser fluence on the post-transfer cell viability in transferring of human colon cell HT-29. It has been observed that: 1) the HT-29 cell viability decreases from 95% to 78% as the laser fluence increases from 258 to 1,500 mJ/cm~2; and 2) cell injury in this study is mainly from the process-induced mechanical stress during the cell droplet formation and cell landing processes while the effects of thermal influence and ultraviolet radiation are below the level of detection.