Optical micro-manipulation of mixed yeast cell populations for analyzing growth behaviour

摘要

We use spatially sculptured light for user-interactive micromanipulation of mixed yeast cell populations toanalyze growth behavioural patterns. There is negligible absorption in the near-infrared region of the lightspectrum making it suitable for direct manipulation of individual cells in a growing population. Rather thanusing a single-beam optical trap, multiple cells are manipulated using a system based on the GeneralizedPhase Contrast (GPC) method, which allows arbitrary trapping configurations i.e. control over the numberof traps, and the size/shape of each trap. This enables the cells to be selectively trapped in all threedimensions(3D) and manipulated in real-time while under direct observation. Here, we impose controlledexperiments using these multiple 3D optical traps to show the alteration of growth patterns in mixed culturesof Saccharomyces cerevisiae and Hanseniaspora uvarum experiencing spatially constrained conditions.