Controlled Release Biomaterials for Directed Embryonic Stem Cell Differentiation

摘要

Controlled release microspheres containing differentiation factors can be incorporated within 3D aggregates of ES cells. This provides us with a method to present signaling molecules in a controlled manner within the microenvironment of differentiating ES cells. Using this technique, the effects of differentiation factor distribution on homogeneity of ES cell differentiation can be examined. Future experiments will utilize real-time RT PCR and immuno-staining with flow cytometry to quantitatively assess the effect of controlled RA presentation on ES cell differentiation.