Surface characterization and in vitro biocompatibility study of surface-sulfonated chitosan membrane

摘要

Chitosan, a kind of glycoaminoglycan, has good biocompatibility and biodegradation, and can be applied as scaffold material in tissue engineering. In addition, blending same low molecular weight glycoaminoglycan such as chondroitin sulfate or keratin in scaffold, can improve cell adhesion and cell proliferation.Therefore imitating these low molecular weight glycoaminoglycan in structure, this study tried to immediately sulfonate the hydroxy groups at C6 of chitosan. At the same time, protection strategy was used to hold the amino groups at C2 of chitosan which was known as the major positive functional group of chitosan with special biological character in cell culture.Surface characterization and in vitro biocompatibility of modified chitosan were evaluated.