Effect of Surface Chemistry on Dendritic Cell Responses and Profile of Carbohydrates Associated with Adsorbed Proteins

摘要

NH_2 and COOH SAM surfaces triggered higher DC activation than CH_3 SAM surfaces. While NH_2 SAM surfaces in general had a higher detectable protein glycosylations compared with CH_3 surfaces, COOH surfaces while activating DCs, did not have similar carbohydrate profiles as NH_2 SAM surfaces. This suggests that different mechanisms may be involved in promoting DC activation, or that other factors such as differential profiles of adsorbed proteins and therefore differential spatial distribution of presented carbohydrates that involve the engagement of different receptor groups, ultimately leading to varying cell responses.