Plant Regeneration of creeping Bluestem (Schizachyrium Scoparium (Michx.) Nash Var. Stoloniferum (Nash) J.Wipff) Via Somatic Embryogenesis

摘要

Currently, most phosphate mine reclamation work (excluding colloidal phosphate settling ponds and gypsum stacks) involves reshaping the landscape and returning overburden, followed by establishment and management of introudced forage crops for pasture. Herbaceous native plants have never been used, except on a small experimetnal basis. Use of native grasses has been further limited because establishment is frequently poor due to the inferior quality of plant material, and seedlings lack vigor and compete poorly with weeds. One objective of our research has been to develop methods for increasing the quantities of suitable propagules for use in mine reclamation. Embryogenic callus growing on solid medium was induced to form preembryos in cell suspension culture, with subsequent maturation (approx 18 weeks) of the embryos on solid medium. Shoots and roots were initiated on solid medium without regulators in 2 to 3 weeks. The optimal medium for further development of the plantlets was on MS + 2,4-D (3 mg/l) +BA (1 mg /L) + GA_3 (1 mg/L) + NAA (0.5 mg/L) + casein hydrolystate (500 mg/L) + 10