RNA POLYMERASE II AS A LINK BETWEEN ALTERNATIVE SPLICING AND CHROMATIN STRUCTURE

摘要

Alternative splicing is a crucial mechanism to generate complexity of the proteome, yet its regulation is an open puzzle. Recent evidence indicates that signature of histone modifications and nucleosome positioning may cause splice site switching, suggesting RNA polymerase IKRNAP II) may mediate the link. Here we present analyses of RNAP II islands and nucleosome enrichment level around skipped exon(SE) 3' splicing sites and constitutive exon(CE) 3' splicing sites(3' ss). We have found histone modification plays more important role in slowing down RNAP II around SE 3' ss in contrast to CE 3' ss. Then we examined whether histone marking around these sites were special, finding that H3K4me3, H4K20mel and H3K27ac marks were different around SE and CE 3' ss in CD4+ T celL.