ELECTROGENERATED AFFINITY POLYMERS FOR THE IMMOBILIZATION OF BIOMOLECULES: FROM MICROBIOSENSORS TO BIOREACTORS

摘要

Electropolymerized films have received considerable attention in the development of biosensors and biochips and are rapidly advancing. This paper reviews the recent advances and the scientific progress in the electrochemical immobilization procedures of biological macromolecules on electrodes via electrogenerated polymer films bearing affinity sites. The biomolecule immobilization was based on the auto-formation of avidin bridges between biotinylated films and biotinylated biomolecules. Some advances in the electrochemical transduction of biological events (enzymatic reactions' or immunoreactions) involving the redox properties, the conductivity or the photochemical properties of electropolymerized films are also presented.