Two-photon excited fluorescence imaging of cell spindles for developmental biology

摘要

By integrating two-photon excited fluorescence (TPEF) technique into confocal optical system, TPEF images of zygote spindles of ICR (Internal Control Region) rats have been obtained in high resolution for the first time. Experimental results demonstrate the validity of TPEF as an efficient probe in investigating cell spindles at early embryonic developmental stage.