Identification of endogenous fluorophores in the layered retina

摘要

In this paper, we measured and analyzed the characteristic of endogenous fluorophores in porcine layered retina by using advanced fluorescence spectroscopy and microscopy imaging technology. It was found that there were obvious contrasts corresponding to the different layers of retina, which may be important for fundus disease diagnosis. The retinal pigment epithelium cells exhibited strong autofluorescence with as emission peak of 600 ± 10nm when excited with 860-nm light. The emission peak of photoreceptors was at 652±5nm, and the emission peak of retinal vessels layer was weak and at 640～700nm, when excited with 488-nm light. Autofluorescence images of three layers of retina were obtained using the same setup. We concluded th'at the main endogenous fluorophore in PRE was lipofuscin and that in retinal vessels was porphyrin. What's more, the FMHW (full width at half, maximum) of retinal fluorescence spectrum was broad, which suggested that there wasn't only one endogenous fluorophores of tissues excited.