Development of Nano-sized Protein Arrays using Block Copolymer Self-Assemblies

摘要

The single molecule approach is potentially of great technological interest as a complementary means to obtain information about protein conformation or molecular interaction. The measurement for molecular interaction in single molecule level requires that the molecule in the question is immobilized by binding to an appropriate surface. W have developed a novel fabrication method of nano-patterned array of bio-molecules with ultra-high density, using self-assembly of block-copolymers. Compared to previous lithographic methods, self-assembly of block-copolymers makes it easier to fabrication of patterns with ～ 10 nanometer feature size and to reduce the number of processing steps. In this work, we have detected a single molecule array on the basis of immunoreaction using protein conjugated gold nanoparticle. In this manner, BCP structure played an important role as confining molecules in single molecule level to a hole avoiding intermolecular interaction or nonspecific binding.