Development of a Novel Real-Time PCR Machine

摘要

A novel multi-channel detection system for real-time PCR machine is proposed in this study. The prototype in this study is equipped with a spectrometer which has the bandwidth of visible light, hence there are more than 6 channels can be detected simultaneously. In contrast to the traditional Real-Time PCR machine with discrete fluorescence wavelength detection channels, the prototype is equipped with continuous fluorescence wavelength detecting ability to multiplex Real-Time PCR. In this study, a HBV DNA template with LC-Red 640 dye and the Internal Control DNA template with LC-Red 705 dye were employed for DNA quantification experiments. The results show that this prototype provides comparable accuracy and reproducibility as the commercial system for DNA quantification with the DNA quantification and detection method proposed in this study.