Probing the Cell Interior with NMR Spectroscopy

摘要

NMR can give more chemical information than any other non-invasive spectroscopic technique for investigating cell biochemistry (Gadian, 1995; Gillies, 1994). The technique can give information about pathway flux in vivo but rarely gives information about the control exerted by an individual enzyme within a pathway. One way of accessing this information is to titrate the activity of the enzyme of interest with a specific irreversible inhibitor.