Characterization of Modified Gold Electrode Sensor with Nanoparticles and Applications

摘要

In the recent development of biosensor, response time of the sensor is drastically improved by using direct coupling of enzymes onto the surface of electrode. The drawback of the direct coupling of enzymes is denature of enzymes on the surface of electrode during the cycles of redox reactionsrnIn order to develop sensors that are useful with predictable performance, the sensors must be well characterized under working conditions so that the performance is ensured.rnWe are developing a biosensor with gold electrode modified by surface coating of cysteamine coupled with nanoparticles, then with hemoglobin (Hb). Due to the high chemical affinity of hemoglobin with nitrite, this Au-cysteamine-nanoparticle-Hb biosensor can be used to detect nitriteitrate by means of a cyclic voltammographic technique.rnThe detection limit of nitriteitrate of this biosensor was well below mM and its response time was almost instantaneous. The reproducibility of performance was tested, and the chemical kinetics of the nitriteitrate redox reaction on the surface of the modified electrode was studied. This sensor was further characterized by the oxidation of peroxide and the results were reported.