Mechanism of Action and Surfactant Influence During Chemotherapy of Brain Tumour Using Doxorubicin-Loaded Poly(butyl Cyanoacrylate) Nanoparticles

摘要

Doxorubicin (Dox) loaded in poly(butyl cyanoacrylate) nanoparticles (PBCA NP) coated with polysorbate 80 or poloxamer 188 produced a high antitumour effect against orthotopic 101/8 glioblastoma in rats. In the present study, the protein adsorption pattern of polysorbate 80- or poloxamer 188-coated DOX-loaded PBCA NP after incubation in rat plasma was investigated. It was shown that the protein adsorption patterns of both types of NP were similar, exhibiting a high adsorption of apolipoprotein ApoA-I (ApoA-I). It is, therefore, hypothesized that the efficacy of these formulations may be related to the interaction of ApoA-I on the particle surface with the scavenger receptor SR-BI expressed at the BBB. Accordingly, these results suggest a correlation between the adsorption of ApoA-I on the nanoparticle surface and the resulting delivery of drugs to the brain by these particles.