'Smart' multivalent fluorescent nanoparticles for cell imaging and drug delivery

摘要

We have synthesized geldanamycin (GA)-polyacrylate coated CdSe/ZnS nanocrystal (NC) conjugates with well-controlled NC/polymer/GA ratios and studied their uptake by HCT116 colon cancer cells. NC-GA conjugates were readily transported into living HCT116 cells with a strong correlation to the GA/NC ratio, while other examined NCs were not, indicating the crucial role of the geldanamycin in the uptake event. This is the first example of dose-responsive drug-mediated uptake of NCs by living cells.