Physico-chemical Structure of Multilayer Polyion Complexes Comprising PolyHydroxylAmine and Genomic DNA

摘要

Alternating multilayers of DNA and polyhydroxylamine [PT] were produced through sequential adsorption onto cationic submicron beads [C]. DNA elution on two enclosed layers and a surface layer of DNA, provided 10μg DNA/mg bead/ per layer and total yield upto 54μg/mg bead. Multilayer polyion complex (MPIC) architecture was probed by dye elution. Cationic sites were lost and gained by DNA and PT addition respectively. Comparison of elution of bound CR anionic dye between bead types reveals cationic site blocking on anionic DNA adsorption of 34 nM/mg bead, and addition on cationic PT adsorption of 14-18nM/mg beads, indicating evidence for a form of layer-by-layer construction.