A NEW APPROACH TO PLASTIC MICROFLUIDIC HYBRIDIZATION ARRAY DEVICES FABRICATION AND IMPROVED HYBRIDIZATION KINETICS WITH SAMPLE OSCILLATION

摘要

DNA probe immobilization on polymeric (e.g. plastic) surface and device integration are both critical to the fabrication of microfluidic hybridization array channel (MHAC) devices. Three oligonucleotide (oligo) probe immobilization procedures were investigated for attaching oligo probes on four different types of plastic surfaces. Utilizing CO_2 IR laser machining and adhesive layer approach, we have developed an improved procedure for realizing a DNA microarray inside a microfluidic channel. This device fabrication procedure allows for more feasible spot placement in the channel and reduced sample adsorption by adhesive tapes used in the fabrication procedure. We also demonstrated the improved hybridization kinetics and increased detection sensitivity in MHAC devices by implementing sample oscillation inside a channel. A limit of detection of 5pM has been achieved in MHAC devices with sample oscillation.