Wide-eld multiphoton imaging with TRAFIX

摘要

Optical approaches have broadened their impact in recent years with innovations in both wide-field and superresolutionimaging, which now underpin biological and medical sciences. Whilst these advances have beenremarkable, to date, the ongoing challenge in optical imaging is to penetrate deeper. TRAFIX is an innovativeapproach that combines temporal focusing illumination with single-pixel detection to obtain wide-field multiphotonimages of uorescent microscopic samples deep through scattering media without correction. It has beenshown that it can image through biological samples such as rat brain or human colon tissue up to a depth ofseven scattering mean-free-path lengths. Comparisons of TRAFIX with standard point-scanning two-photonmicroscopy show that the former can yield a five-fold higher signal-to-background ratio while significantly reducingphotobleaching of the specimen. Here, we show the first preliminary demonstration of TRAFIX withthree-photon excitation imaging dielectric beads. We discuss the advantages of the TRAFIX approach combinedwith compressive sensing for biomedicine.