Surface Modified Chitosan Tissue Engineering Scaffolds for Biomimetic Periosteum on Cortical Bone Allografts

摘要

We report new approaches to developing biomimetic periosteum. Chitosan has previously been shown to be biocompatible for a variety of skeletal tissue engineering applications. The scaffold morphologies on allografts shown in Fig. 1 have feature sizes suitable for bone tissue engineering. Chitosan NF were directly electrospun onto the diaphyseal surface of allografts thus enabling a thin tissue engineered scaffold to be produced on the cortical bone surface. Further PEMs deposition of TMC and heparin are confirmed by XPS analysis (appearance of sulfate at 168 eV and ammonium at 402 eV after addition of TMC-heparin PEMs). Our group has also previously shown TMC and heparin PEMs possess the ability to bind and stabilize heparin binding growth factors.3 This allows our tissue engineered scaffolds to bind important growth factors such as FGF-2, which may play a part in healing large segmental defects in cortical bone.