Production of Mycoprotein by Candida utilis in Solid-state Fermentation Using Jatropha curcas Seed Cake as Substrate

摘要

Jatropha curcas (physic nut) has drawn attention in recent years as a source of seed oil that can provide an economically viable substitute for diesel. However, there is very little work on viable approach for use of the oil-extracted Jatropha curcas seed cake (JCSC) so far to the best of our knowledge. This article reports our studies on the utilization of this huge biomass by solid-state fermentation (SSF) for the production of mycoprotein. Candida utilis strain with high mycoprotein yield was purchased and used for fermentation. Maximum mycoprotein production (35.26 percent of substrate) was observed at 75% substrate moisture and a growth period of 3 days. Compared to JCSC without fermentation, the crude protein (CP) in JCSC with SSF was increased by 48.65%, and the total amino acids (TAA) and essential amino acids (EAA) contents were raised by 17.60% and 18.73%,respectively. Especially, the levels of valine, lysine and isoleucine were increased greatly (P<0.05). The in vitro CP digestibility of fermented JCSC was raised by 35.83%. These results demonstrated that the nutritive value of JCSC was improved significantly after SSF by Candida utilis.