Rapid Protein Production in CHO Cells: From Transfection to 100g in 6 Weeks

摘要

To meet the increasing demand for proteins supporting biologies discovery, and shorten overall IND enabling biologies development timeline, a rapid protein expression system using CHO-K1 cells has been developed at WuXi Biologies: specifically up to 500 mg of proteins can be delivered in 3-4 weeks by transient production; while 100-200 g of proteins can be delivered 6 weeks after transfection which we call WuXian Express. The poster will describe this system and compare key antibody product quality attributes from materials generated from WuXian Express, stable pools and Master Cell Banks (MCBs), and demonstrate comparability among them. Materials generated from this system have also been used at WuXi Biologies for developability study during antibody candidate molecule selection, early downstream, formulation and analytical method development, saving about 2 months compared with materials generated from stable pools.