Nanoelectrodes on Screen Printed Substrate for Sensing Cholinesterase Inhibitors

摘要

In this work gold and platinum nanoelectrode ensembles (NEEs) have been produced with a quite controlled pore diameter range using a polycarbonate templating membrane with pore size between 10 and 50 nm. Such nanostructured surfaces have been used in a novel hybrid electrochemical device, by coupling NEEs with disposable screen printed substrate (NEEs-SPS). Electrodes responses to hydrogen peroxide and glucose have been collected and compared with other glucose-oxidase sensors. NEEs-SPS device based on acetyl cholinesterase (AcChE) has been applied for the first time in detection of cholinesterase in form of inhibition biosensor. As a specific substrate of the main reaction acetyl thiocholine chloride salt (AcTCh~+Cl~-) has been used. Analytical performances have been evaluated in term of sensor sensitivity and linearity of response. A measurement protocol has been adopted for detecting specific organo-phosphorous compounds, using Dichlorvos as a pesticide reference. Preliminary results on fruit sample are also reported and discussed.