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Characterization of Chitin and Its Complexes Extracted from Natural Raw Sources

机译:甲壳素的特征及其复合物从天然原料源提取

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It is known that the main source of chitin and chitosan are shells of shrimp and other sea crustaceans. Alternative row sources of chitin, chitosan and its complexes are the lowest plants - mushrooms and insects. Also industrial wastes, especially from brewing of beer and manufacture of wine and ethanol can be used for extracting chitosan-containing products. The present research is aimed to the extraction of chitin and its complexes from alternative row sources, such as insects (cockroaches Pariplaneta Americana linnaeus and bees Apis mellifera lineaus), mushrooms (Amanita phalloides and Lactarius subdulius), waste banana wine (Kovibar and Urwibutso Inc.) and beer products (Bralirwa Inc., traditional sorghum) characteristic for Rwanda and their characterization using FTIR spectroscopy and elementary analysis, In chitin and its complexes extraction from all used raw sources, conditions for deproteinization were: 8% NaOH at 95 °C for 1 h and demineralization involved treatment with 6.7 % HC1 at room temperature .Chitin and its complexes in the extracted samples were identified by FTIR spectroscopy using reference sample of Aspegillus niger mushrooms. The presence of chitin parts causes the absorption band at 1650, 1552 and 1376 cm~(-1), which correspond to vibrations of amide groups ?amide I?, ?amide II? and ?amide III?, respectively. Using elemental analysis, the ratios of chitin and glucan parts were estimated and the percentage of chitin composition of all species was determined. For most of raw sources a fraction of chitin part was greater than that of glucan part. The chitin content of the samples studied ranged between 0.7-0.8 % of DM (dried mass) for wine (beer) waste products and 38% of dried mass (DM) for cockroaches.
机译:众所周知,甲壳素和壳聚糖的主要来源是虾和其他海上甲壳类动物的壳。替代行壳酸,壳聚糖及其复合物是植物和昆虫最低的植物。工业废物,特别是酿造啤酒和葡萄酒和乙醇的制造可用于提取含壳聚糖的产品。目前的研究旨在从替代排源中提取甲壳素及其复合物,例如昆虫(蟑螂Pariplaneta American Linnaeus和Bees Apis Mellifera Lineaus),蘑菇(amanita phalloides和Lactarius subdulius),废香蕉葡萄酒(Kovibar和Urwibutso Inc 。对于1小时而脱矿质,在室温下用6.7%HCl治疗.Chitin及其在提取的样品中的复合物通过FTIR光谱法使用Aspegillus Niger蘑菇的参考样品鉴定出来的。几丁质部件的存在导致1650,1552和1376cm〜(-1)的吸收带,其对应于酰胺基团的振动?酰胺I?,amide II?和?酰胺III?。利用元素分析,估计了几丁质和葡聚糖部分的比例,测定了所有物种的几丁质组合物的百分比。对于大多数原始来源,几丁质部件大于葡聚糖部分的一部分。样品的甲壳素含量在葡萄酒(啤酒)废物(啤酒)废物(啤酒)废物中的0.7-0.8%的DM(干酪)之间的范围为38%的蟑螂干含量(DM)。

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