Photodynamic Therapy Application of PAMAM-Porphyrin Molecule on Stomach Cancer Cells

摘要

In this study, effect of a novel LED-based light source developed for 96-well-plates cell culture applications, was tried on AGS stomach cancer cell line, in combination with Poly(amido amine) (PAMAM) modified - porhyrin molecule. For each 4 generation of modified PpⅨ molecule 5 different concentrations were tried. According to results PAMAM molecule did not show photosensitizer property also didn't show any toxic effect even if higher concentrations. Morphology and real time monitoring analysis results supported each other and confirmed that, PpⅨ molecules with and without modificated high concentrations (＞100μM) caused cell death via toxicicity. Optimal concentrations for PAMAM modified Pp-Ⅸ was found 50 μM and 100 μM.