Determination of Total Alkaloids in Different Parts of Actinidia arguta by Spectrophotography

摘要

Total Alkaloids are important active materials of Actinidia arguta. However, no study has been conducted to explore the content determination method of the Actinidia arguta total alkaloid. An effective method was established in order to determine the total alkaloids from different parts of Actinidia arguta. Using ultrasonic cleaner, the total alkaloids were extracted from the samples and tested at 415nm with the spectroscopy by the means of acid dye method. The calibration curve of aconitine were linear (r=0.9997), and the recovery rate was prefect (98.9%). The results of measurement were consistent in 3 hours. The content of alkaloids in roots was the highest, with 1.25mg/g, followed by leaves and fruits, the least were stems. The method is simple and effective, and the contents of total alkaloids were different in different parts of Actinidia arguta.