Solid-Phase Microextraction Fiber Coating for Biological Analysis

摘要

A procedure for solid-phase microextraction fiber preparation was introduced, which combined a quartz fiber substrate with a blend of SiO_2 sol-gel and PVA, as a way of increasing both the adhesive ability and the extracting capability of fibers. Double cross-linked network of organic and inorganic layer was prepared for the fiber coatings by repeated freezing-thawing. The effects of salt on fiber coating composition and microextraction conditions were investigated. The relative standard deviations (RSD) (n=3) were obtained between 6.9% and 10.2%. The fiber coatings for biological molecule's extraction of soyasaponin were presented.