Determination of axial fluorophore distributions without strong focusing apertures using noncollinear optical parametric amplification

摘要

Techniques like confocal or two-photon microscopy are nowadays well known and established to sample a three-dimensional distribution of fluorophores [1]. Those have in common that data acquisition for a lateral and axial resolution is achieved using high numerical aperture optics. However, this approach cannot be applied for systems with limited aperture such as the human eye. Measuring the lifetime and axially resolved fluorescence from the human retina opens the way for a new ophthalmologic diagnosis such as age-related macular degeneration [2].