Quantitative phase imaging (QPI) is an important tool in biomedicine that yields label-free access to cellular andsubcellular structures with nanometer-scale sensitivity. However, implementation of QPI involves a transmissionbasedgeometry and requires thin samples, severely hindering its overall utility in biomedicine. Here we describe ourrecently developed method, quantitative oblique back illumination microscopy (qOBM), which overcomes thissignificant limitation and achieves epi-mode, tomographic, quantitative phase imaging in thick samples, includingintact thick tissues. Here we describe the method in detail, show validation experiments and results from thickscattering samples.
展开▼
