Experimental Study on Oil-degradation Microbial Consortium from Oil-contaminated Soil by Different Enrichment and Domestication

摘要

In order to increase the effectiveness of remediation on oil-contaminated soil, culture techniques and molecular biological methods were adopted to analyze community structure enriched and domesticated by different ways. The results showed that the bacterial concentration cultured in a medium of inorganic salts and oil was 1.30×l0~(12)cfu/ml, while the bacterial concentration cultured in a medium of LB and oil was 3.05×10~8cfu/ml; Within the perspective of structure, the microbial community cultured in a medium of inorganic salts and oil contained 11 kinds of bacterial, Meanwhile, the microbial community cultured in a medium of LB and oil was composed of 4 kinds of bacteria. Based on the results of this study, it can be inferred that the cultured medium has a significant influence on the composition of microbial communities. When domesticated oil-contaminated soil was cultured in a medium of inorganic salts and oil, the microbial concentrations and diversities were relatively higher than those cultured in LB and oil.