The Response of Antioxidant Enzyme and ATPase in Bacteria Exposed to 1,2-dichlorobenzene

摘要

The activity response of the antioxidant enzymes superoxide dismutase(SOD), catalase(CAT), ATP enzyme activities of ESCHERICHIA COLI Q8, BACILLUS SUBTILIS L11, and BACILLUS CEREUS OL-1 following exposure to 1,2-dichlorobenzene(1,2-DCB) was investigated. The bacterial strains were treated with the different concentrations of 1,2-DCB. Results obtained indicated that SOD and CAT activities in the tested bacteria increased significantly in a concentration-dependent manner after different concentrations of 1,2-DCB were applied. The activity of SOD in B. SUBTILIS was stimulated and reached the highest level after treatment with 10 mg/L 1,2-DCB for 3 h. For B.CEREUS OL-1, there was another stimulation of SOD activity after 1,2-DCB application for about 5 h The stimulation by 1,2-DCB showed a relative lag for E. COLI. 1,2-DCB had an evident influence on ATPase activity in the three bacteria within a relatively short period. 1,2-DCB would have caused a certain oxidative stress on the three bacteria which may not only elevate SOD and CAT activities but also generate new SOD isozymes to antagonize oxidative stress. All indirectly reflect the existence of poisonous and harmful material in the environment, and can indicate the influence of pollution sensitivily. Therefore SOD, CAT and ATP enzyme activity in microbial body can be regarded as a molecular index of polluting, which is feasible.