Absorption Enhancing Effect of Sodium Dodecyl Sulfate on Metformin Hydrochloride in Rat Colon

摘要

The aim of this study was to observe the absorption enhancing effect of sodium dodecyl sulfate on metformin hydrochloride in colon of rat. Using in vivo intestinal loop model in rat while the ileum was took as blank group and colon as the experiment groups with different concentration of sodium dodecyl sulfate (1%, 2%, 4%). The colon/ileum ratio of the absorption rate constant of metformin hydrochloride was evaluated through calculating the residual dose of circulating solution in presupposition time points. Intergroup absorption rate constant and the rising percent of the absorption rate constant were different significantly (P＜O.05). The absorption rate constant of colon were -0.22±0.03, -0.37+0.06, -0.89±0.09, -0.8610.05 ug h~(-1)-cm~(-1) (n=6) and the rising percent of the constant absorption value were 68.66 + 8.28%, 304.88 + 28.76%, 293.75 ± 33.19% (n = 6), respectively. The result showed that the absorption of metformin hydrochloride was increased with the concentration of sodium dodecyl sulfate. However, the absorption rate constant reached maxium when the concentration of sodium dodecyl sulfate was 2%, this may be because the circulating metformin hydrochloride solution could be more viscous which affect the absorption of metformin hydrochloride when sodium dodecyl sulfate was raised, In conclusion, the absorption of metformin hydrochloride can be promoted by sodium dodecyl sulfate in the colon of rat and this can provide biophamaceutics data for novel pharmaceutical preparation.