Stem Tissue Culture of Pinus elliottii× Pinus caribaea

摘要

Using tender stems of the current-year in 5-year-old P. lliottii × P. caribaea as explants and exploiting orthogonal xperiments, we optimized the growth conditions of Pinus elliottii Pinus caribaea in vitro and successfully obtained the plantlets. e found that: (1) as explants, fragments at 1.5~3.0 cm from top o bottom of stems that cultured in DCR media supplemented ith 2.0 mg/L N6-benzyladenine (6-BA), 0.10 mg/L alphanaphthyl cetic acid (NAA) and 30 g/L sucrose have the best arly differentiation rate of 43.7%; (2) among the tested onditions, DCR media supplemented with 2.5 mg/L 6-BA and.2 mg/L NAA is most suitable for propagation of adventitious uds giving a propagation rate and fold of 96.7% and 6.2, espectively; (3) DCR media supplemented with 0.3 mg/L NAA,.5 g/L activated carbon and 20 g/L sucrose is best for elongation f adventitious buds giving propagation rate of 444.6%; (4) ransplantation of seedlings to ? DCR media supplemented with.0 mg/L NAA and cultured in dark for 10 days followed by llumination 12 h/d with 40 μmol·m-2·s-1 intensity resulted as igh as 55.3% rooting rate.