Preparation and Characterization of Mn_(0.5)Zn_(0.5)Fe_2O_4@Au Composite Nanoparticles and Its Anti-Tumor Effect on Glioma Cells

摘要

To explore the preparation method and characters of a new gold nanoshells on maganese-zinc ferrite(Mn_(0.5)Zn_(0.5)Fe_2O_4@Au) composite nanoparticles. Mn_(0.5)Zn_(0.5)Fe_2O_4@Au nanoparticles with core/shell structure were synthesized by reduction of Au~(3+) with trisodium citrate in the presence of Mn_(0.5)Zn_(0.5)Fe_2O_4 magnetic nanoparticles(MZF-NPs) prepared by improved co-preciption with the character of superparamagnetism and detected by transmission electron microscopy(TEM), scanning electron microscopy(SEM), x-ray diffraction(XRD), energy dispersive spectrometry(EDS) and Marven laser particle size analyzer.Thermodynamic test was used to observe temperature change of various doses of Mn_(0.5)Zn_(0.5)Fe_2O_4@Au nanoparticles. The cytotoxicity of the Mn_(0.5)Zn_(0.5)Fe_2O_4@Au composite nanoparticles in vitro was tested by the MTT assay. The therapeutic effect of Mn_(0.5)Zn_(0.5)Fe_2O_4@Au composite nanoparticles combined with magnetic fluid hyperthermia (MFH) on human glioma cells were evaluated in vitro by an MTT assay.The results indicated that the Mn_(0.5)Zn_(0.5)Fe_2O_4@Au composite nanoparticles were prepared successfully. The core/shell particles were spherical with exact average diameter of them was 66.9nm.EDS showed each Mn_(0.5)Zn_(0.5)Fe_2O_4@Au nanoparticle contained Mn, Zn, Fe, O and Au elements, and this proved Au had successfully attached to Mn_(0.5)Zn_(0.5)Fe_2O_4.The result of thermodynamic test showed that Mn_(0.5)Zn_(0.5)Fe_2O_4@Au composite nanoparticles could serve as a heating source under alternating magnetic field(AMF) exposure leading to reach their steady temperature(40-45□). Moreover, Mn_(0.5)Zn_(0.5)Fe_2O_4@Au composite nanoparticles didn't show cytotoxicity in vitro. The therapeutic result reveals that Mn_(0.5)Zn_(0.5)Fe_2O_4@Au composite nanoparticles can significantly inhibit the growth of glioma cells.The conclusion was that the self-prepared Mn_(0.5)Zn_(0.5)Fe_2O_4@Au composite nanoparticles had strong magnetic responsiveness and good power absorption capabilities in the high frequency AMF,then they could suggested to be useful for glioma hyperthemia. Mn_(0.5)Zn_(0.5)Fe_2O_4@Au composite nanoparticles can not only be directed to tumor region in a given magnetic field more exactly but also produce marked thermotherapy.