Immobilization mediated enhancement of Phyllanthin and Hypophyllanthin from Phyllanthus amarus

摘要

Phyllanthus amarus plant is used in traditional system of medicine as hepatoprotective drug for which the major lignans phyllanthin and hypophyllanthin are responsible. As so far no significant work has been done on culture aspect of this plant, hence realizing the hepatoprotective potential, the present investigation was undertaken. A cost effective process was developed for enhancing phyllanthin and hypophyllanthin utilizing immobilization technique. HPTLC was used to compare phyllanthin and hypophyllanthin contents in calcium alginate immobilized cells obtained from fresh grown plants and M.S. Medium was supplemented with different abiotic elicitors, for treatment with chitosan, copper sulphate, phenylalanine and silver nitrate solution to make whole process commercially viable. It was revealed that silver nitrate and phenylalanine in low concentration enhances phyllanthin and hypophyllanthin yield as compared to control. The study revealed that increase in the content of phyllanthin and hypophyllanthin was elicitor concentration dependent and silver nitrate treatment give maximum yield of hepatoprotective bioactives as compared to other abiotic elicitors used.