Characterization of mechano-sensitive nano-containers for targeted vasodilation

摘要

Cardiovascular diseases are the worldwide number one cause of mortality. The blood flow in diseased human coronary arteries differs from the blood flow in the healthy vessels. This fact should be used for designing targeted localized delivery of vasodilators with a purely physical drug release trigger. Thus, we have proposed mechano-sensitive liposomes as mechano-sensitive containers. One has to tailor the liposome's properties, so that containers are stable under physiological conditions in health, but release their cargo near the constricted vessels at body temperature. In order to determine the shear stress threshold for release, both the morphology of the healthy and diseased human arteries and the mechanical property of the liposomes have to be known. We have shown that micro computed tomography (μCT) techniques allow visualizing the lumen of human coronary arteries and provide the basis for flow simulations to extract the wall shear stress of healthy and stenosed regions in human coronary arteries. The behavior of the mechano-sensitive liposomes is currently investigated by means of microfluidics and spatially resolved small-angle X-ray scattering. The liposomes are injected into micro-channels mimicking in vivo situation. The scattering signal from the liposomes reveals information about their size, shape, and wall thickness.