Detection of endothelial cell-derived nitric oxide release using gold-coated carbon electrode modified with nafion: the tests in control and diabetic rats

摘要

In the present study, the gold-coated carbon electrodes were fabricated for in vivo detection endothelial cells-derived nitric oxide release. To improve sensitivity and selectivity for the detection of acetylcholine-mediated vascular nitric oxide release, the electrode surface was modified with tetra-sulfonated nickel phthalocyanine (NiTSPc) and nafion. The experiments showed that gold-coated carbon electrode modified with nafion is able to detect nitric oxide in vivo for both groups of control and diabetic rats. Moreover, the results also indicated that the ratio of endothelial cell-derived nitric oxide release after acetylcholine activation in controls and in diabetes was 12:1 when using electrochemical detection.