High sensitive volumetric imaging of renal microcirculation in vivo using Ultrahigh Sensitive Optical Microangiography

摘要

We present a non-invasive, label-free imaging technique called Ultrahigh Sensitive Optical Microangiography (UHS-OMAG) for high sensitive volumetric imaging of renal microcirculation. The UHS-OMAG imaging system is based onspectral domain optical coherence tomography (SD-OCT), which uses a 47000 A-line scan rate CCD camera to performan imaging speed of 150 frames per second that takes only ～7 seconds to acquire a 3D image. The technique, capable ofmeasuring slow blood flow down to 4 um/s, is sensitive enough to image capillary networks, such as peritubularcapillaries and glomerulus within renal cortex. We show superior performance of UHS-OMAG in providing depth-resolved volumetric images of rich renal microcirculation. We monitored the dynamics of renal microvasculature duringrenal ischemia and reperfusion. Obvious reduction of renal microvascular density due to renal ischemia was visualizedand quantitatively analyzed. This technique can be helpful for the assessment of chronic kidney disease (CKD) whichrelates to abnormal microvasculature.