Electrically Controlled H-PDLC Array Modulated Multi-frequencies Division Multiplexed Fluorescence Confocal Microscopy

摘要

A novel two channel multi-frequencies division multiplexed confocal fluorescence microscopy (FDMCF) system based on the holographic polymer dispersed liquid crystal (H-PDLC) optical chopper modulation is reported. The characteristic of unique FDMCF system is that within the FDMCF system, the multi-channel exciter laser beams are modulated with different carrying frequencies, after the fluorescence signals are collected, and through data analyzing process, the FDMCF system can realize the multiply points parallel detection synchronously with high temporal and spatial resolution. Besides, combined with the electrically controlled H-PDLC grating array working as the optical chopper to replace the mechanical chopper, it can improve and develop the FDMCF system from two channels to unlimited system. The paper has done the experiments to demonstrate the two-channel FDMCF system based on H-PDLC modulation and analysis the benefits from the electrically-controlled integrated electro-optical H-PDLC devices.