Quantitative in-vivo lifetime imaging using a time-domain platform with a supercontinuum tunable laser for extended spectral coverage

摘要

The continuously expanding number of fluorescent probes developed for molecular imaging in vivo requires new instruments, more flexible and with higher quantification power. Responding to those requirements we propose a new instrument: it combines the sensitivity of time correlated single photon detection with the extended spectral coverage of a pulsed supercontinuum laser in a sensitive and flexible time-domain platform for in vivo molecular imaging in small animals. The performance of the system is demonstrated on a case study, using NEO-STEM-PMSR50- PEG fluorescent silica nanoprobes and sequential imaging of CD-1 nude mice.