Study and Application on Separation and Enrichment of Calcium-teracycline Complex by Aqueous Two-phase Gas Floatation Spectrometry

摘要

A novel and green method for separating and enriching trace TC in environment water by Aqueous Two-Phase Gas Floatation (ATGF) Spectrophotometry has been proposed. The method estimated by HPLC was good, which has many excellences such as simple equipment, speedy detection, high sensitiveness, easy separation from organic solvent and not leaving any infectant. In this paper, the hydrophobic complex composed of Ca(II) and TC was floated into organic phase under the optimal conditions: pH=8, the floatation equipment is home-made, tetrahytrofuran as organic solvent, sodium chloride as separating phase reagent. The data were obtained by spectrophotometry after floatation. The linear regression equation is A=2.032×105C–0.0393, linear ranges is from 3.4×10-7 to 8.3×10-5 mol L-1, respectively, with the correlation coefficient (r) better than 0.9999, The average relative recoveries is 99.8% ,The limit of detection is 2.43×10-7 mol L-1 ,This method was applied to analyse the trace TC in the water, the result is good.