Evaluation of Chitosan Oligosaccharide Nanoparticles Prepared by A Novel Microemulsion-Coacervation Method

摘要

@@Chitosan oligosaccharide（COS）is the low molecular weight chitosan, which is more readily soluble than chitosan. Both chitosan and COS are biodegradable, biocompatible, and low-toxic materials, which are very popular in drug delivery system, especially as materials for developing micro/nanoparticles. The present approach for preparing chitosan nanoparticles, however, still have some drawbacks, such as low encapsulation rate, high toxicity induced by abundant emulsifier and cross linking agents. Herein, a novel microemulsion-coacervation technique was developed to prepare non-cross-linked chitosan oligosaccharides nanoparticles(COS-NPs). This technique is an improvement of emulsion cross-linking way which often used to prepare chitosan–based microspheres. The major improvement is avoiding toxicity from abundant emulsifier by adding co-emulsifier and glutaraldehyde as chemical crosslinker. Furthermore, the obtained nanoparticles were characterized and evaluated by its drug release mechanism, in vitro cytotoxicity and in vivo tissue distribution.