FT-IR spectrometry utilization for determining changes in erythrocyte susceptibility to oxidative stress

摘要

We tested the hypothesis that FT-IR spectrometry was useful for determining oxidative stress damage on erythrocytes. Endurance-trained subjects performed a standardized endurance-training session at 75% of maximal oxygen consumption each week over 19 consecutive weeks. Capillary blood samples were taken before and after test-sessions and plasma and erythrocytes were separately analyzed using Fourier-transform infrared spectrometry. Exercise-induced change in plasma concentrations and erythrocyte IR absorptivities ( C-H_n of fatty acyl moieties, C=O and N-H of proteins, P=O of phospholipids, COO~- of amino-acids, and C-O of lactate) were monitored and compared to training level. First training weeks induced normalization of plasma concentration changes during exercise (unchanged for glucose, moderately increased for lactate, higher increases for triglycerides, glycerol, and fatty acids) while erythrocyte phospholipids alteration remained elevated (P < 0.05). Further, training reduced the exercise-induced erythrocyte lactate content increase ( C-O; P < 0.05) and phospholipids alteration ( C-H_n and P=O; P < 0.05) during exercise. These changes paralleled the lowering of exercise-induced hemoconcentration (P < 0.05) and plasma lactate concentration increase during exercise (P < 0.05). These correlated changes between plasma and erythrocyte parameters suggest that hemoconcentration and lactate acidosis (plasmatic and intracellular) are important factors contributing to reduce erythrocyte susceptibility to oxidative stress during chronic endurance training.