Analysis of the diversity of ammonia oxidizing and denitrifying bacteria in submerged filter biofilms for the treatment of urban wastewater by temperature gradient gel electrophoresis

摘要

Wastewater treatment by means of fixed biofilm technology is one of the major biotechnologi-cal processes used worldwide. In this study, nitrogen removal from wastewater is achieved by a combination of nitrification and denitrification in a submerged filter biofilm. The system was very efficient for the removal of organic matter, and significantly reduced N in the effluent water. The spatial and temporal diversity of ammonia oxidizing and denitrifying bacteria in biofilms of the treatment plant was estimated by a temperature-gradient gel electrophoresis (TGGE) approach.