Gene Expression Differences of TNBG on Human Hepatocellular Carcinoma Cell Line QGY-7701 with cDNA Microarray

摘要

To further investigate the mechanism of TNBG. We used cDNA microarray to explore Gene Expression Difference of TNBG on human hepatocellular carcinoma cell line QGY-7701. After treated with TNBG for 3 days, mRNA from both control and treated cells were isolated and purified to prepare the probes, then reversely transcribed to cDNA with the incorporation of fluore-scent labelled dUTP. The probes were hybridized with a microarray representing the 8464 human cDNAs. The fluorescent signals of Cy3 and Cy5 were scanned and analyzed. We found that the gene expression profiles were changed greatly. Among the 8464 target genes, 1200(14.2%) different expression genes were detected. The 654 genes were down-regulated and the 546 genes were up-regulated. They were functionally related to cell proliferation cycle, replication, metabolism, and so on. The expression difference of some genes involved in lipid biosynthesis, lipid transport, and lipid degradation were extremely significant.