Catchment volume and penetration depth in different suspensions of liver mitochondria monitored in scattering chamber

摘要

Spectrometry is a well established method for in vitro and in vivo measurements. For interpretation of experimental results and patient monitoring it is important to regard the catchment volume and penetration depth of backscattered light. We investigated different suspensions of rat liver mitochondria as well as rat liver homogenate in a scattering chamber combined with a scanning procedure. To compare these results with a common used medium we measured Lipofundin 2% additionately. The highest light intensity caused Lipofundin 2%, followed by 100% mitochondrial concentration, liver homogenate and 50% mitochondrial concentration. Most of times the light penetrates slightly pronounced in lateral than in forward direction. Furthermore, we investigated the dependence of optical fibers with different diameter on catchment volume and penetration depth. Optical fibers were applied with a diameter of 200 μm and 250 μm respectively presenting a great increase in catchment volume and penetration depth in the larger one.